Before beginning with this protocol, ensure that you have enough quantities of age- and sex-matched Handle and genetically modified mice from the right mouse pressure. Deliver only one-stained cell Handle with the Fixable Viability Dye by heat-killing a hundred μL of one-stained cell Handle sample as explained in steps 12c https://csgohealth.com/how-ozone-generators-can-improve-indoor-air-quality/